mouse anti ox 42  (Bio-Rad)

Journal: Frontiers in Aging Neuroscience

Article Title: Spinal glial cell derived extra-pituitary prolactin contributes to postoperative pain in females

doi: 10.3389/fnagi.2026.1741102

Figure Lengend Snippet: PRL expression in the spinal cord of sham- and incision-operated female rats and mice. (A, B) PRL/OX-42 (A) and PRL/NeuN co-labeling in the ipsilateral spinal cord (SC) of female rats 1 day after sham. Cyan arrows mark PRL + astrocyte-like cells. NeuN was used as a pan-neuronal marker. Scale bars: 50 μm. (C, C′) PRL labeling in SC 1 day after sham and incision (INC) procedure. Images show dorsal horn regions (laminae I–V; partial ventral laminae). Images were captured with a 10 × objective (A, B, C, C′) . (D) PRL + density in the ipsilateral dorsal horn of SC 1 day after sham and incision (INC) procedure. (E, E′) Immunolabeling of PRL in laminae I–II of the ipsilateral spinal cord (SC) from female mice 1 day after sham surgery (Sham). Yellow arrow indicates OX42 + microglia, and pink arrows indicate PRL − /GFAP + astrocytes. (F, F′) PRL labeling in laminae I–II of the ipsilateral SC from female mice 1 day after incision (INC). Yellow arrow indicates OX42 + microglia, and cyan arrows indicate PRL + /GFAP + astrocytes. Surgical procedures are indicated in row titles. Antibodies and corresponding fluorescence colors are shown in the photomicrographs. Images were captured with a 40 × objective. Scale bar: 25 μm for all panels (E-F′) . (G) Percentages of PRL + cells in NeuN + , Ox-42 + and GFAP + in female mice 1 days after INC.

Article Snippet: The following antibodies were used in the study: PRL protein in rats was detected with rabbit anti-PRL (Agilent-DAKO, Santa Clara, CA; cat: A0569; 1:100) ( ); PRL in mice was detected with rabbit anti-PRL (Bioss, Boston, MA; cat: BS-23763R; 1:200); pSTAT5 in rats was detected with rabbit anti-pSTAT5 (Tyr694, Cell Signaling Technology, Beverly, MA; cat: 9314S; 1:200) ( ); rat DRG and spinal cord neurons were detected with mouse monoclonal anti-NeuN antibodies (Millipore-Sigma, St. Louis, MO; catalog MAB377; 1:100); mouse spinal astrocytes and Schwann cells in hind paw were labeled with chicken anti-GFAP (Neuromics, Edina, MN; catalog CH22102; 1:100); and rat and mouse monocytes/macrophages/dendritic cells/microglia were labeled with mouse monoclonal antibody OX-42 (CD11b/c; Bio-Rad, Hercules, CA; catalog MCA275GA; 1:50) ( ).

Techniques: Expressing, Labeling, Marker, Immunolabeling, Fluorescence

Journal: bioRxiv

Article Title: Targeting PD-L1 in solid cancer with myeloid cells expressing a CAR-like immune receptor

doi: 10.1101/2024.01.29.577873

Figure Lengend Snippet: Non-modified-(Ctrl), CARIR-Δz, or CARIR-z-engineered THP-1 effector cells were co-cultured in the presence of 5ng/ml PMA for 3 days with RM-1 hPD-L1 or WT-RM-1 target cells at effector to target ratio of 5:1. Following the co-culture, the cells were stained with APC anti-human CD11b, Brilliant Violet 605 anti-human PD-L1, and Zombie NIR viability dye. The number of the remaining live CD11b - target cells following the co-culture was quantified by flow cytometry with the use of absolute counting beads. A . Representative contour plots show the percentage change for PD-L1 + , PD-L1 int , and PD-L1 - RM-1 hPD-L1 tumor cells following the co-culture. B . BAR graphs summarize the percentage data shown on panel A. C . BAR graphs summarize the absolute cell count data shown on panel A. D . Representative contour plots show the percentage change of RM-1 tumor cells (PD-L1 - ) following the co-culture. E . BAR graph summarizes the percentage data shown on panel D. F . BAR graph summarizes the absolute cell count data shown on panel D. Tumor cells were gated on non-beads, live, singlets, and CD11b - . Data were presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by one-way ANOVA.

Article Snippet: The following monoclonal antibodies (mAbs) and their isotype controls were used for flow cytometry: APC human PD-1, APC human PD-L1, Brilliant Violet human PD-L1, APC human CD86, APC human CD11b, PE human CD247 (CD3z), APC mouse CD11b; PE human EGFR from R&D Systems; APC mouse PD-L1 from Tonbo Biosciences.

Techniques: Modification, Cell Culture, Co-Culture Assay, Staining, Flow Cytometry, Cell Counting